Review



human flt3 ligand  (Proteintech)


Bioz Verified Symbol Proteintech is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Proteintech human flt3 ligand
    Human Flt3 Ligand, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+flt3+ligand/Animal-free+Recombinant+Human+FLT3+Ligand/pm41809821-60-57-59
    Average 93 stars, based on 12 article reviews
    human flt3 ligand - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Centrifugation:

    Article Title: cGAS/STING-mediated upregulation of NKG2D ligands in LSCs contributes to enhanced sensitivity to NK cells.
    Article Snippet: .. Briefly, mononuclear cells were separated from BM by density-gradient centrifugation with Ficoll-Hypaque (Cytiva, Uppsala, Sweden), and cultured in IMDM (HyClone, Logan, Utah, USA) supplemented with 15% FBS (Gibco, Australia), 4 mM L-Glutamine (Gibco, Brazil), 100 ng/mL human IL-3 (Proteintech, China), 10 ng/mL human IL-6 (Proteintech, China), 50 ng/mL human M-CSF (Proteintech, China), TPO (Proteintech, China), 50 ng/mL human FLT3-ligand (Proteintech, China), 50 ng/mL human SCF (Proteintech, China) and 1% penicillin and streptomycin. .. Human peripheral blood mononuclear cells (PBMCs) were firstly separated by density-gradient centrifugation with FicollHypaque (Cytiva, Uppsala, Sweden) from healthy donors, and then primary NK cells were isolated from PBMC by human NK Cell Isolation Kit (Cat#: 130-092-657, Miltenyi Biotec) via untouched negative MACS Separation as instructions.

    Article Title: cGAS/STING-mediated upregulation of NKG2D ligands in LSCs contributes to enhanced sensitivity to NK cells
    Article Snippet: .. Briefly, mononuclear cells were separated from BM by density-gradient centrifugation with Ficoll-Hypaque (Cytiva, Uppsala, Sweden), and cultured in IMDM (HyClone, Logan, Utah, USA) supplemented with 15% FBS (Gibco, Australia), 4 mM L-Glutamine (Gibco, Brazil), 100 ng/mL human IL-3 (Proteintech, China), 10 ng/mL human IL-6 (Proteintech, China), 50 ng/mL human M-CSF (Proteintech, China), TPO (Proteintech, China), 50 ng/mL human FLT3-ligand (Proteintech, China), 50 ng/mL human SCF (Proteintech, China) and 1% penicillin and streptomycin. .. Human peripheral blood mononuclear cells (PBMCs) were firstly separated by density-gradient centrifugation with Ficoll-Hypaque (Cytiva, Uppsala, Sweden) from healthy donors, and then primary NK cells were isolated from PBMC by human NK Cell Isolation Kit (Cat#: 130-092-657, Miltenyi Biotec) via untouched negative MACS Separation as instructions.

    Cell Culture:

    Article Title: cGAS/STING-mediated upregulation of NKG2D ligands in LSCs contributes to enhanced sensitivity to NK cells.
    Article Snippet: .. Briefly, mononuclear cells were separated from BM by density-gradient centrifugation with Ficoll-Hypaque (Cytiva, Uppsala, Sweden), and cultured in IMDM (HyClone, Logan, Utah, USA) supplemented with 15% FBS (Gibco, Australia), 4 mM L-Glutamine (Gibco, Brazil), 100 ng/mL human IL-3 (Proteintech, China), 10 ng/mL human IL-6 (Proteintech, China), 50 ng/mL human M-CSF (Proteintech, China), TPO (Proteintech, China), 50 ng/mL human FLT3-ligand (Proteintech, China), 50 ng/mL human SCF (Proteintech, China) and 1% penicillin and streptomycin. .. Human peripheral blood mononuclear cells (PBMCs) were firstly separated by density-gradient centrifugation with FicollHypaque (Cytiva, Uppsala, Sweden) from healthy donors, and then primary NK cells were isolated from PBMC by human NK Cell Isolation Kit (Cat#: 130-092-657, Miltenyi Biotec) via untouched negative MACS Separation as instructions.

    Article Title: β-Catenin interacts with canonical RBPs including MSI2 to associate with a Wnt signalling mRNA network in myeloid leukaemia cells.
    Article Snippet: Cell culture and drug treatments The myeloid cell lines K562, HL60, HEL, U937, PLB-985, NOMO1, OCI-AML3, EOLI, ML-1, THP-1, KU812 (The European Collection of Authenticated Cell Cultures) and OCI-AML2, MV4-11, KG-1, KG1a SET2, NB4 and MONOMAC6 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) were confirmed mycoplasma-free and authenticated via short-tandem repeat (STR) analysis prior to general culture as previously [30]. β-Catenin was stabilised using the GSK-3β inhibitor CHIR99021 (Merck-Millipore) as previously described [8], whilst transcription was inhibited via 2 μg/ml Actinomycin D (Merck-Millipore) at the stated timepoints. .. Purified human CB CD34+ HSPC were isolated and cultured at 5 × 105/mL in StemSpan SFEMII (StemCell Technologies) supplemented with human stem cell factor (150 ng/mL), human FLT3-ligand (150 ng/mL), and human thrombopoietin (TPO; 20 ng/mL; Proteintech Group). .. HSPC number and subsequent fold expansion in liquid culture were ascertained by manual cell counting in combination with trypan blue (Merck-Millipore) exclusion assays.

    Article Title: cGAS/STING-mediated upregulation of NKG2D ligands in LSCs contributes to enhanced sensitivity to NK cells
    Article Snippet: .. Briefly, mononuclear cells were separated from BM by density-gradient centrifugation with Ficoll-Hypaque (Cytiva, Uppsala, Sweden), and cultured in IMDM (HyClone, Logan, Utah, USA) supplemented with 15% FBS (Gibco, Australia), 4 mM L-Glutamine (Gibco, Brazil), 100 ng/mL human IL-3 (Proteintech, China), 10 ng/mL human IL-6 (Proteintech, China), 50 ng/mL human M-CSF (Proteintech, China), TPO (Proteintech, China), 50 ng/mL human FLT3-ligand (Proteintech, China), 50 ng/mL human SCF (Proteintech, China) and 1% penicillin and streptomycin. .. Human peripheral blood mononuclear cells (PBMCs) were firstly separated by density-gradient centrifugation with Ficoll-Hypaque (Cytiva, Uppsala, Sweden) from healthy donors, and then primary NK cells were isolated from PBMC by human NK Cell Isolation Kit (Cat#: 130-092-657, Miltenyi Biotec) via untouched negative MACS Separation as instructions.

    Article Title: β-Catenin interacts with canonical RBPs including MSI2 to associate with a Wnt signalling mRNA network in myeloid leukaemia cells
    Article Snippet: The myeloid cell lines K562, HL60, HEL, U937, PLB-985, NOMO1, OCI-AML3, EOLI, ML-1, THP-1, KU812 (The European Collection of Authenticated Cell Cultures) and OCI-AML2, MV4-11, KG-1, KG1a SET2, NB4 and MONOMAC6 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) were confirmed mycoplasma-free and authenticated via short-tandem repeat (STR) analysis prior to general culture as previously [ ]. β-Catenin was stabilised using the GSK-3β inhibitor CHIR99021 (Merck-Millipore) as previously described [ ], whilst transcription was inhibited via 2 μg/ml Actinomycin D (Merck-Millipore) at the stated timepoints. .. Purified human CB CD34 + HSPC were isolated and cultured at 5 × 10 5 /mL in StemSpan SFEMII (StemCell Technologies) supplemented with human stem cell factor (150 ng/mL), human FLT3-ligand (150 ng/mL), and human thrombopoietin (TPO; 20 ng/mL; Proteintech Group). .. HSPC number and subsequent fold expansion in liquid culture were ascertained by manual cell counting in combination with trypan blue (Merck-Millipore) exclusion assays.

    Purification:

    Article Title: β-Catenin interacts with canonical RBPs including MSI2 to associate with a Wnt signalling mRNA network in myeloid leukaemia cells.
    Article Snippet: Cell culture and drug treatments The myeloid cell lines K562, HL60, HEL, U937, PLB-985, NOMO1, OCI-AML3, EOLI, ML-1, THP-1, KU812 (The European Collection of Authenticated Cell Cultures) and OCI-AML2, MV4-11, KG-1, KG1a SET2, NB4 and MONOMAC6 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) were confirmed mycoplasma-free and authenticated via short-tandem repeat (STR) analysis prior to general culture as previously [30]. β-Catenin was stabilised using the GSK-3β inhibitor CHIR99021 (Merck-Millipore) as previously described [8], whilst transcription was inhibited via 2 μg/ml Actinomycin D (Merck-Millipore) at the stated timepoints. .. Purified human CB CD34+ HSPC were isolated and cultured at 5 × 105/mL in StemSpan SFEMII (StemCell Technologies) supplemented with human stem cell factor (150 ng/mL), human FLT3-ligand (150 ng/mL), and human thrombopoietin (TPO; 20 ng/mL; Proteintech Group). .. HSPC number and subsequent fold expansion in liquid culture were ascertained by manual cell counting in combination with trypan blue (Merck-Millipore) exclusion assays.

    Article Title: β-Catenin interacts with canonical RBPs including MSI2 to associate with a Wnt signalling mRNA network in myeloid leukaemia cells
    Article Snippet: The myeloid cell lines K562, HL60, HEL, U937, PLB-985, NOMO1, OCI-AML3, EOLI, ML-1, THP-1, KU812 (The European Collection of Authenticated Cell Cultures) and OCI-AML2, MV4-11, KG-1, KG1a SET2, NB4 and MONOMAC6 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) were confirmed mycoplasma-free and authenticated via short-tandem repeat (STR) analysis prior to general culture as previously [ ]. β-Catenin was stabilised using the GSK-3β inhibitor CHIR99021 (Merck-Millipore) as previously described [ ], whilst transcription was inhibited via 2 μg/ml Actinomycin D (Merck-Millipore) at the stated timepoints. .. Purified human CB CD34 + HSPC were isolated and cultured at 5 × 10 5 /mL in StemSpan SFEMII (StemCell Technologies) supplemented with human stem cell factor (150 ng/mL), human FLT3-ligand (150 ng/mL), and human thrombopoietin (TPO; 20 ng/mL; Proteintech Group). .. HSPC number and subsequent fold expansion in liquid culture were ascertained by manual cell counting in combination with trypan blue (Merck-Millipore) exclusion assays.

    Isolation:

    Article Title: β-Catenin interacts with canonical RBPs including MSI2 to associate with a Wnt signalling mRNA network in myeloid leukaemia cells.
    Article Snippet: Cell culture and drug treatments The myeloid cell lines K562, HL60, HEL, U937, PLB-985, NOMO1, OCI-AML3, EOLI, ML-1, THP-1, KU812 (The European Collection of Authenticated Cell Cultures) and OCI-AML2, MV4-11, KG-1, KG1a SET2, NB4 and MONOMAC6 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) were confirmed mycoplasma-free and authenticated via short-tandem repeat (STR) analysis prior to general culture as previously [30]. β-Catenin was stabilised using the GSK-3β inhibitor CHIR99021 (Merck-Millipore) as previously described [8], whilst transcription was inhibited via 2 μg/ml Actinomycin D (Merck-Millipore) at the stated timepoints. .. Purified human CB CD34+ HSPC were isolated and cultured at 5 × 105/mL in StemSpan SFEMII (StemCell Technologies) supplemented with human stem cell factor (150 ng/mL), human FLT3-ligand (150 ng/mL), and human thrombopoietin (TPO; 20 ng/mL; Proteintech Group). .. HSPC number and subsequent fold expansion in liquid culture were ascertained by manual cell counting in combination with trypan blue (Merck-Millipore) exclusion assays.

    Article Title: β-Catenin interacts with canonical RBPs including MSI2 to associate with a Wnt signalling mRNA network in myeloid leukaemia cells
    Article Snippet: The myeloid cell lines K562, HL60, HEL, U937, PLB-985, NOMO1, OCI-AML3, EOLI, ML-1, THP-1, KU812 (The European Collection of Authenticated Cell Cultures) and OCI-AML2, MV4-11, KG-1, KG1a SET2, NB4 and MONOMAC6 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) were confirmed mycoplasma-free and authenticated via short-tandem repeat (STR) analysis prior to general culture as previously [ ]. β-Catenin was stabilised using the GSK-3β inhibitor CHIR99021 (Merck-Millipore) as previously described [ ], whilst transcription was inhibited via 2 μg/ml Actinomycin D (Merck-Millipore) at the stated timepoints. .. Purified human CB CD34 + HSPC were isolated and cultured at 5 × 10 5 /mL in StemSpan SFEMII (StemCell Technologies) supplemented with human stem cell factor (150 ng/mL), human FLT3-ligand (150 ng/mL), and human thrombopoietin (TPO; 20 ng/mL; Proteintech Group). .. HSPC number and subsequent fold expansion in liquid culture were ascertained by manual cell counting in combination with trypan blue (Merck-Millipore) exclusion assays.



    Similar Products

    96
    Miltenyi Biotec recombinant human flt3 ligand miltenyi biotec
    Recombinant Human Flt3 Ligand Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+flt3+ligand/Human+IL-7%2C+research+grade/pm42486103-554-30-33
    Average 96 stars, based on 1 article reviews
    recombinant human flt3 ligand miltenyi biotec - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    95
    Miltenyi Biotec flt3 l
    Flt3 L, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+flt3+ligand/Human+Flt3-Ligand%2C+premium+grade/10__1016_slash_j__isci__2026__116736-656-33-34
    Average 95 stars, based on 1 article reviews
    flt3 l - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    95
    Miltenyi Biotec human fms like tyrosine kinase ligand hflt3 ligand
    Human Fms Like Tyrosine Kinase Ligand Hflt3 Ligand, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+flt3+ligand/Human+Flt3-Ligand%2C+premium+grade/bio_rxiv__64898__2026__07__07__736903-359-31-37
    Average 95 stars, based on 1 article reviews
    human fms like tyrosine kinase ligand hflt3 ligand - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    95
    Miltenyi Biotec flt3l
    Flt3l, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+flt3+ligand/Human+Flt3-Ligand%2C+premium+grade/10__1080_slash_2162402x__2026__2695692-52-40-42
    Average 95 stars, based on 1 article reviews
    flt3l - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    95
    Miltenyi Biotec flt3 ligand
    Flt3 Ligand, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+flt3+ligand/Human+Flt3-Ligand%2C+premium+grade/pm42312423-485-0-5
    Average 95 stars, based on 1 article reviews
    flt3 ligand - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    95
    Miltenyi Biotec hflt3 l
    Hflt3 L, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+flt3+ligand/Human+Flt3-Ligand%2C+premium+grade/bio_rxiv__64898__2026__06__15__732298-57-49-51
    Average 95 stars, based on 1 article reviews
    hflt3 l - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    95
    Miltenyi Biotec human flt3l
    (A) Scheme of synapse formation between murine <t>Flt3L-differentiated</t> bone marrow-derived DCs (BMDC) and OT-I T cells. LPS: lipopolysaccharide; CpG-B: oligodeoxynucleotide; OVA: ovalbumin; IC: immune complex; SIINFEKL: ovalbumin (257-264) peptide epitope; PFA: paraformaldehyde. (B) Representative confocal microscopy images of the BMDC-T cell synapses immunostained for cis-Golgi marker GM-130 (magenta), γ-tubulin (yellow), DAPI (blue) and α-tubulin (green). White arrowheads: MTOC polarization. Scale bar, 10 µm. (C) Scheme of quantification of MTOC polarization: the distance between the MTOC and the DC-T cell interface (a) was divided by the diameter of the DC (b). (D) Quantification of MTOC polarization with unstimulated BMDCs or TLR ligand-stimulated BMDCs unloaded or loaded with OVA, OVA-Immunocomplexes or SIINFEKL. For each of the 3 mouse cultures, 30-35 cells were analysed. Mean ± standard error of the mean, Kruskal-Wallis test, Dunn’s multiple comparison **P<0.01, ****P<0.0001 (E) Same as panel D, but now displayed as percentage DCs with polarized MTOC (defined as polarization index < 0.3) for individual mice. (F) Electron micrographs of two different immunological synapses between T cell and BMDC, showing MTOC, Golgi apparatus (GA), and immunological synapse (IS); Black boxes indicate magnified areas. Scale bars, 1 µm.
    Human Flt3l, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+flt3+ligand/Human+Flt3-Ligand%2C+premium+grade/bio_rxiv__64898__2026__05__13__724773-180-43-46
    Average 95 stars, based on 1 article reviews
    human flt3l - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    93
    Proteintech human flt3 ligand
    (A) Scheme of synapse formation between murine <t>Flt3L-differentiated</t> bone marrow-derived DCs (BMDC) and OT-I T cells. LPS: lipopolysaccharide; CpG-B: oligodeoxynucleotide; OVA: ovalbumin; IC: immune complex; SIINFEKL: ovalbumin (257-264) peptide epitope; PFA: paraformaldehyde. (B) Representative confocal microscopy images of the BMDC-T cell synapses immunostained for cis-Golgi marker GM-130 (magenta), γ-tubulin (yellow), DAPI (blue) and α-tubulin (green). White arrowheads: MTOC polarization. Scale bar, 10 µm. (C) Scheme of quantification of MTOC polarization: the distance between the MTOC and the DC-T cell interface (a) was divided by the diameter of the DC (b). (D) Quantification of MTOC polarization with unstimulated BMDCs or TLR ligand-stimulated BMDCs unloaded or loaded with OVA, OVA-Immunocomplexes or SIINFEKL. For each of the 3 mouse cultures, 30-35 cells were analysed. Mean ± standard error of the mean, Kruskal-Wallis test, Dunn’s multiple comparison **P<0.01, ****P<0.0001 (E) Same as panel D, but now displayed as percentage DCs with polarized MTOC (defined as polarization index < 0.3) for individual mice. (F) Electron micrographs of two different immunological synapses between T cell and BMDC, showing MTOC, Golgi apparatus (GA), and immunological synapse (IS); Black boxes indicate magnified areas. Scale bars, 1 µm.
    Human Flt3 Ligand, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+flt3+ligand/Animal-free+Recombinant+Human+FLT3+Ligand/pm41809821-60-57-59
    Average 93 stars, based on 1 article reviews
    human flt3 ligand - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    (A) Scheme of synapse formation between murine Flt3L-differentiated bone marrow-derived DCs (BMDC) and OT-I T cells. LPS: lipopolysaccharide; CpG-B: oligodeoxynucleotide; OVA: ovalbumin; IC: immune complex; SIINFEKL: ovalbumin (257-264) peptide epitope; PFA: paraformaldehyde. (B) Representative confocal microscopy images of the BMDC-T cell synapses immunostained for cis-Golgi marker GM-130 (magenta), γ-tubulin (yellow), DAPI (blue) and α-tubulin (green). White arrowheads: MTOC polarization. Scale bar, 10 µm. (C) Scheme of quantification of MTOC polarization: the distance between the MTOC and the DC-T cell interface (a) was divided by the diameter of the DC (b). (D) Quantification of MTOC polarization with unstimulated BMDCs or TLR ligand-stimulated BMDCs unloaded or loaded with OVA, OVA-Immunocomplexes or SIINFEKL. For each of the 3 mouse cultures, 30-35 cells were analysed. Mean ± standard error of the mean, Kruskal-Wallis test, Dunn’s multiple comparison **P<0.01, ****P<0.0001 (E) Same as panel D, but now displayed as percentage DCs with polarized MTOC (defined as polarization index < 0.3) for individual mice. (F) Electron micrographs of two different immunological synapses between T cell and BMDC, showing MTOC, Golgi apparatus (GA), and immunological synapse (IS); Black boxes indicate magnified areas. Scale bars, 1 µm.

    Journal: bioRxiv

    Article Title: T cells compete via reverse MHC class I signaling at the synapse with dendritic cells to secure Golgi recruitment for activation

    doi: 10.64898/2026.05.13.724773

    Figure Lengend Snippet: (A) Scheme of synapse formation between murine Flt3L-differentiated bone marrow-derived DCs (BMDC) and OT-I T cells. LPS: lipopolysaccharide; CpG-B: oligodeoxynucleotide; OVA: ovalbumin; IC: immune complex; SIINFEKL: ovalbumin (257-264) peptide epitope; PFA: paraformaldehyde. (B) Representative confocal microscopy images of the BMDC-T cell synapses immunostained for cis-Golgi marker GM-130 (magenta), γ-tubulin (yellow), DAPI (blue) and α-tubulin (green). White arrowheads: MTOC polarization. Scale bar, 10 µm. (C) Scheme of quantification of MTOC polarization: the distance between the MTOC and the DC-T cell interface (a) was divided by the diameter of the DC (b). (D) Quantification of MTOC polarization with unstimulated BMDCs or TLR ligand-stimulated BMDCs unloaded or loaded with OVA, OVA-Immunocomplexes or SIINFEKL. For each of the 3 mouse cultures, 30-35 cells were analysed. Mean ± standard error of the mean, Kruskal-Wallis test, Dunn’s multiple comparison **P<0.01, ****P<0.0001 (E) Same as panel D, but now displayed as percentage DCs with polarized MTOC (defined as polarization index < 0.3) for individual mice. (F) Electron micrographs of two different immunological synapses between T cell and BMDC, showing MTOC, Golgi apparatus (GA), and immunological synapse (IS); Black boxes indicate magnified areas. Scale bars, 1 µm.

    Article Snippet: From the femurs and tibias, bone marrow derived stem cells were obtained and cultured in RPMI-1640 medium with 10% fetal bovine serum (FBS, 758093, Greiner bio-one), 1% antibiotic-antimycotic (15240-062, Gibco), 1% ultraglutamine (BE17-605E/U1, Lonza Bioscience), 50 μM β-mercaptoethanol (60-24-2, Sigma-Aldrich) and 200 ng/ml human Flt3L (130-096-479, Miltenyi Biotec) for eight days at 37oC in an incubator with 10% CO 2 .

    Techniques: Derivative Assay, Confocal Microscopy, Marker, Comparison

    (A) Scheme of MTOC polarization by antibody- or protein domain-coated bead stimulation of murine Flt3L-differentiated bone marrow-derived DCs (BMDCs). LPS: lipopolysaccharide; CpG-B: oligodeoxynucleotide; PFA: paraformaldehyde. (B) Representative immunofluorescence images of the BMDC-bead conjugates. Cells were immunostained for cis-Golgi marker GM-130 (magenta), γ-tubulin (yellow), DAPI (blue) and α-tubulin (green). Beads were coated with isotype or antibody against MHC class I. Straight outline: T cell; dotted outline BMDC; white arrowhead: MTOC polarization. Scale bar, 10 µm. (C) Quantification of MTOC polarization in BMDCs incubated with beads coated with antibodies against MHC class I, MHC class II, or ICAM-1, or with LFA-I or CD28 protein domains. One-way ANOVA Kruskal-Wallis test with Dunn’s Multiple Comparisons. (D) Quantification of MTOC polarization in BMDCs incubated with beads coated with antibodies against MHC class I treated with nocodazole inhibitor targeting the microtubules (Mt), the Src-I1 and PP1 inhibitors targeting tyrosine kinase Src, and the phospholipase C inhibitor (U73122; U22) and its negative control compound (U73343; U43) and the PKC inhibitor Gö6983. One-way ANOVA with Dunnett’s multiple comparison, Mean ± standard error of the mean (**P<0.01; ****P<0.0001).

    Journal: bioRxiv

    Article Title: T cells compete via reverse MHC class I signaling at the synapse with dendritic cells to secure Golgi recruitment for activation

    doi: 10.64898/2026.05.13.724773

    Figure Lengend Snippet: (A) Scheme of MTOC polarization by antibody- or protein domain-coated bead stimulation of murine Flt3L-differentiated bone marrow-derived DCs (BMDCs). LPS: lipopolysaccharide; CpG-B: oligodeoxynucleotide; PFA: paraformaldehyde. (B) Representative immunofluorescence images of the BMDC-bead conjugates. Cells were immunostained for cis-Golgi marker GM-130 (magenta), γ-tubulin (yellow), DAPI (blue) and α-tubulin (green). Beads were coated with isotype or antibody against MHC class I. Straight outline: T cell; dotted outline BMDC; white arrowhead: MTOC polarization. Scale bar, 10 µm. (C) Quantification of MTOC polarization in BMDCs incubated with beads coated with antibodies against MHC class I, MHC class II, or ICAM-1, or with LFA-I or CD28 protein domains. One-way ANOVA Kruskal-Wallis test with Dunn’s Multiple Comparisons. (D) Quantification of MTOC polarization in BMDCs incubated with beads coated with antibodies against MHC class I treated with nocodazole inhibitor targeting the microtubules (Mt), the Src-I1 and PP1 inhibitors targeting tyrosine kinase Src, and the phospholipase C inhibitor (U73122; U22) and its negative control compound (U73343; U43) and the PKC inhibitor Gö6983. One-way ANOVA with Dunnett’s multiple comparison, Mean ± standard error of the mean (**P<0.01; ****P<0.0001).

    Article Snippet: From the femurs and tibias, bone marrow derived stem cells were obtained and cultured in RPMI-1640 medium with 10% fetal bovine serum (FBS, 758093, Greiner bio-one), 1% antibiotic-antimycotic (15240-062, Gibco), 1% ultraglutamine (BE17-605E/U1, Lonza Bioscience), 50 μM β-mercaptoethanol (60-24-2, Sigma-Aldrich) and 200 ng/ml human Flt3L (130-096-479, Miltenyi Biotec) for eight days at 37oC in an incubator with 10% CO 2 .

    Techniques: Derivative Assay, Immunofluorescence, Marker, Incubation, Negative Control, Comparison