human flt3 ligand (Proteintech)
Structured Review
Human Flt3 Ligand, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+flt3+ligand/Animal-free+Recombinant+Human+FLT3+Ligand/pm41809821-60-57-59
Average 93 stars, based on 12 article reviews
Images
Related Articles
Centrifugation:Article Title: cGAS/STING-mediated upregulation of NKG2D ligands in LSCs contributes to enhanced sensitivity to NK cells. Article Snippet: .. Briefly, mononuclear cells were separated from BM by density-gradient centrifugation with Ficoll-Hypaque (Cytiva, Uppsala, Sweden), and cultured in IMDM (HyClone, Logan, Utah, USA) supplemented with 15% FBS (Gibco, Australia), 4 mM L-Glutamine (Gibco, Brazil), 100 ng/mL human IL-3 (Proteintech, China), 10 ng/mL human IL-6 (Proteintech, China), 50 ng/mL human M-CSF (Proteintech, China), TPO (Proteintech, China), 50 ng/mL Article Title: cGAS/STING-mediated upregulation of NKG2D ligands in LSCs contributes to enhanced sensitivity to NK cells Article Snippet: .. Briefly, mononuclear cells were separated from BM by density-gradient centrifugation with Ficoll-Hypaque (Cytiva, Uppsala, Sweden), and cultured in IMDM (HyClone, Logan, Utah, USA) supplemented with 15% FBS (Gibco, Australia), 4 mM L-Glutamine (Gibco, Brazil), 100 ng/mL human IL-3 (Proteintech, China), 10 ng/mL human IL-6 (Proteintech, China), 50 ng/mL human M-CSF (Proteintech, China), TPO (Proteintech, China), 50 ng/mL Cell Culture:Article Title: cGAS/STING-mediated upregulation of NKG2D ligands in LSCs contributes to enhanced sensitivity to NK cells. Article Snippet: .. Briefly, mononuclear cells were separated from BM by density-gradient centrifugation with Ficoll-Hypaque (Cytiva, Uppsala, Sweden), and cultured in IMDM (HyClone, Logan, Utah, USA) supplemented with 15% FBS (Gibco, Australia), 4 mM L-Glutamine (Gibco, Brazil), 100 ng/mL human IL-3 (Proteintech, China), 10 ng/mL human IL-6 (Proteintech, China), 50 ng/mL human M-CSF (Proteintech, China), TPO (Proteintech, China), 50 ng/mL Article Title: β-Catenin interacts with canonical RBPs including MSI2 to associate with a Wnt signalling mRNA network in myeloid leukaemia cells. Article Snippet: Cell culture and drug treatments The myeloid cell lines K562, HL60, HEL, U937, PLB-985, NOMO1, OCI-AML3, EOLI, ML-1, THP-1, KU812 (The European Collection of Authenticated Cell Cultures) and OCI-AML2, MV4-11, KG-1, KG1a SET2, NB4 and MONOMAC6 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) were confirmed mycoplasma-free and authenticated via short-tandem repeat (STR) analysis prior to general culture as previously [30]. β-Catenin was stabilised using the GSK-3β inhibitor CHIR99021 (Merck-Millipore) as previously described [8], whilst transcription was inhibited via 2 μg/ml Actinomycin D (Merck-Millipore) at the stated timepoints. .. Purified human CB CD34+ HSPC were isolated and cultured at 5 × 105/mL in StemSpan SFEMII (StemCell Technologies) supplemented with human stem cell factor (150 ng/mL), Article Title: cGAS/STING-mediated upregulation of NKG2D ligands in LSCs contributes to enhanced sensitivity to NK cells Article Snippet: .. Briefly, mononuclear cells were separated from BM by density-gradient centrifugation with Ficoll-Hypaque (Cytiva, Uppsala, Sweden), and cultured in IMDM (HyClone, Logan, Utah, USA) supplemented with 15% FBS (Gibco, Australia), 4 mM L-Glutamine (Gibco, Brazil), 100 ng/mL human IL-3 (Proteintech, China), 10 ng/mL human IL-6 (Proteintech, China), 50 ng/mL human M-CSF (Proteintech, China), TPO (Proteintech, China), 50 ng/mL Article Title: β-Catenin interacts with canonical RBPs including MSI2 to associate with a Wnt signalling mRNA network in myeloid leukaemia cells Article Snippet: The myeloid cell lines K562, HL60, HEL, U937, PLB-985, NOMO1, OCI-AML3, EOLI, ML-1, THP-1, KU812 (The European Collection of Authenticated Cell Cultures) and OCI-AML2, MV4-11, KG-1, KG1a SET2, NB4 and MONOMAC6 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) were confirmed mycoplasma-free and authenticated via short-tandem repeat (STR) analysis prior to general culture as previously [ ]. β-Catenin was stabilised using the GSK-3β inhibitor CHIR99021 (Merck-Millipore) as previously described [ ], whilst transcription was inhibited via 2 μg/ml Actinomycin D (Merck-Millipore) at the stated timepoints. .. Purified human CB CD34 + HSPC were isolated and cultured at 5 × 10 5 /mL in StemSpan SFEMII (StemCell Technologies) supplemented with human stem cell factor (150 ng/mL), Purification:Article Title: β-Catenin interacts with canonical RBPs including MSI2 to associate with a Wnt signalling mRNA network in myeloid leukaemia cells. Article Snippet: Cell culture and drug treatments The myeloid cell lines K562, HL60, HEL, U937, PLB-985, NOMO1, OCI-AML3, EOLI, ML-1, THP-1, KU812 (The European Collection of Authenticated Cell Cultures) and OCI-AML2, MV4-11, KG-1, KG1a SET2, NB4 and MONOMAC6 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) were confirmed mycoplasma-free and authenticated via short-tandem repeat (STR) analysis prior to general culture as previously [30]. β-Catenin was stabilised using the GSK-3β inhibitor CHIR99021 (Merck-Millipore) as previously described [8], whilst transcription was inhibited via 2 μg/ml Actinomycin D (Merck-Millipore) at the stated timepoints. .. Purified human CB CD34+ HSPC were isolated and cultured at 5 × 105/mL in StemSpan SFEMII (StemCell Technologies) supplemented with human stem cell factor (150 ng/mL), Article Title: β-Catenin interacts with canonical RBPs including MSI2 to associate with a Wnt signalling mRNA network in myeloid leukaemia cells Article Snippet: The myeloid cell lines K562, HL60, HEL, U937, PLB-985, NOMO1, OCI-AML3, EOLI, ML-1, THP-1, KU812 (The European Collection of Authenticated Cell Cultures) and OCI-AML2, MV4-11, KG-1, KG1a SET2, NB4 and MONOMAC6 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) were confirmed mycoplasma-free and authenticated via short-tandem repeat (STR) analysis prior to general culture as previously [ ]. β-Catenin was stabilised using the GSK-3β inhibitor CHIR99021 (Merck-Millipore) as previously described [ ], whilst transcription was inhibited via 2 μg/ml Actinomycin D (Merck-Millipore) at the stated timepoints. .. Purified human CB CD34 + HSPC were isolated and cultured at 5 × 10 5 /mL in StemSpan SFEMII (StemCell Technologies) supplemented with human stem cell factor (150 ng/mL), Isolation:Article Title: β-Catenin interacts with canonical RBPs including MSI2 to associate with a Wnt signalling mRNA network in myeloid leukaemia cells. Article Snippet: Cell culture and drug treatments The myeloid cell lines K562, HL60, HEL, U937, PLB-985, NOMO1, OCI-AML3, EOLI, ML-1, THP-1, KU812 (The European Collection of Authenticated Cell Cultures) and OCI-AML2, MV4-11, KG-1, KG1a SET2, NB4 and MONOMAC6 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) were confirmed mycoplasma-free and authenticated via short-tandem repeat (STR) analysis prior to general culture as previously [30]. β-Catenin was stabilised using the GSK-3β inhibitor CHIR99021 (Merck-Millipore) as previously described [8], whilst transcription was inhibited via 2 μg/ml Actinomycin D (Merck-Millipore) at the stated timepoints. .. Purified human CB CD34+ HSPC were isolated and cultured at 5 × 105/mL in StemSpan SFEMII (StemCell Technologies) supplemented with human stem cell factor (150 ng/mL), Article Title: β-Catenin interacts with canonical RBPs including MSI2 to associate with a Wnt signalling mRNA network in myeloid leukaemia cells Article Snippet: The myeloid cell lines K562, HL60, HEL, U937, PLB-985, NOMO1, OCI-AML3, EOLI, ML-1, THP-1, KU812 (The European Collection of Authenticated Cell Cultures) and OCI-AML2, MV4-11, KG-1, KG1a SET2, NB4 and MONOMAC6 (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) were confirmed mycoplasma-free and authenticated via short-tandem repeat (STR) analysis prior to general culture as previously [ ]. β-Catenin was stabilised using the GSK-3β inhibitor CHIR99021 (Merck-Millipore) as previously described [ ], whilst transcription was inhibited via 2 μg/ml Actinomycin D (Merck-Millipore) at the stated timepoints. .. Purified human CB CD34 + HSPC were isolated and cultured at 5 × 10 5 /mL in StemSpan SFEMII (StemCell Technologies) supplemented with human stem cell factor (150 ng/mL), |
